Western blot analysis of Cathepsin Z in immunoprecipitation pellets from WI-38 lysate immunoprecipitated using ab182575 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Cathepsin Z with ab182575 at 1/80 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/5000 dilution + WI-38 lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 20 µg
All lanes : Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/2000 dilutionLane 1 : Human fetal liver lysateLane 2 : HeLa lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution