All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilutionLane 1 : A549 lysateLane 2 : HeLa lysateLane 3 : SW480 lysateLane 4 : fetal kidney lysateLane 5 : Human small intestine lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).