All lanes : Anti-Cathepsin V antibody [BV55-1] (ab115232) at 1/1000 dilutionLane 1 : Human liver whole tissue lysateLane 2 : HeLa whole cell lysateLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Human testis tissue (formalin-fixed, paraffin-embedded) stained with ab115232 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.