Anti-Cathepsin L antibody [33/2]
| Name | Anti-Cathepsin L antibody [33/2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6314 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 33/2 |
| Applications | WB ELISA IHC-F ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Full length native protein (purified) (Human lung cancer line EPLC 32 M1) |
| Description | Mouse Monoclonal |
| Gene | CTSL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Cathepsin L staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6314, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![All lanes : Anti-Cathepsin L antibody [33/2] (ab6314) at 1/1000 dilution (3% milk block)Lane 1 : Lung (Human) Tissue LysateLane 2 : Lung (Mouse) Tissue Lysate Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Kidney (Mouse) Tissue LysateLane 5 : Kidney (Rat) Tissue Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21656_Cathepsin-L-Primary-antibodies-ab6314-13.jpg)
All lanes : Anti-Cathepsin L antibody [33/2] (ab6314) at 1/1000 dilution (3% milk block)Lane 1 : Lung (Human) Tissue LysateLane 2 : Lung (Mouse) Tissue Lysate Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Kidney (Mouse) Tissue LysateLane 5 : Kidney (Rat) Tissue Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Anti-Cathepsin L antibody [33/2] (ab6314) at 1/750 dilution + whole cell lysate prepared from U87MG human glioblastoma at 50 µgSecondaryGoat anti-mouse HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21657_Cathepsin-L-Primary-antibodies-ab6314-6.jpg)
Anti-Cathepsin L antibody [33/2] (ab6314) at 1/750 dilution + whole cell lysate prepared from U87MG human glioblastoma at 50 µgSecondaryGoat anti-mouse HRP at 1/2000 dilution
ab6314 at a 1/150 dilution staining Cathepsin L in CHO cells by Immunocytochemistry/ Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab6314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6314, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21659_Cathepsin-L-Primary-antibodies-ab6314-15.jpg)
Overlay histogram showing HeLa cells stained with ab6314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6314, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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