Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab134169 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescence staining of MCF7 cells with purified ab134169 at a working dilution of 1 in 50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab134169 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

All lanes : Anti-Cathepsin D antibody [EPR3054] (ab134169) at 1/2000 dilution (unpurified)Lane 1 : MCF7 cell lysateLane 2 : A431 cell lysateLane 3 : SKBR3 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human breast ductal infiltrating carcinoma tissue, staining Cathepsin D using unpurified ab134169 at a 1/250 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD