Anti-Catalase antibody [1A1]
| Name | Anti-Catalase antibody [1A1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16771 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 1A1 |
| Applications | IP ELISA ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | CAT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab16771 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16771, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunoprecipitation analysis of HeLa cell lysates with ab16771. Lane 1: Input. Lane 2: Precipitates.
![Overlay histogram showing HeLa cells stained with ab16771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21492_Catalase-Primary-antibodies-ab16771-3.jpg)
Overlay histogram showing HeLa cells stained with ab16771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 - A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1
Zhang J, Kim J, Alexander A, Cai S, Tripathi DN, Dere R, Tee AR, Tait-Mulder J, Di Nardo A, Han JM, Kwiatkowski E, Dunlop EA, Dodd KM, Folkerth RD, Faust PL, Kastan MB, Sahin M, Walker CL. Nat Cell Biol. 2013 Oct;15(10):1186-96.
Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines - Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines
Baarine M, Ragot K, Genin EC, El Hajj H, Trompier D, Andreoletti P, Ghandour MS, Menetrier F, Cherkaoui-Malki M, Savary S, Lizard G. J Neurochem. 2009 Oct;111(1):119-31.