Anti-Caspase-14 antibody [EPR12927]

Name	Anti-Caspase-14 antibody [EPR12927]
Supplier	Abcam
Catalog	ab174847
Prices	$387.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR12927
Applications	WB IHC-P FC IP ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Description	Rabbit Monoclonal
Gene	CASP14
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : ECV-304 cell lysateLane 3 : HACAT cell lysateLane 4 : Human skin lysateLysates/proteins at 10 µg per lane.

Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.

Immunohistochemical analysis of paraffin-embedded fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.

Flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1:10 dilution (red), or rabbit IgG negative control (green).

Western blot analysis on immunoprecipitation pellet from fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.