Immunohistochemical analysis of formalin-fixed and paraffin-embedded human rectal carcinoma tissue anti-ompF (primary antibody dilution at 1:200)
IHC-P of human rectal carcinoma tissue using ompF (dilution at 1:200)
Lane 1, ladder; lanes 2,3&4, wild type (BW 25113) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively); Lanes 5,6&7, ΔOmpF (JW0912) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively).
10% SDS- PAGE followed by a Western blot: lane 1, ladder; lanes 2,3&4, wild type( BW 25113) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively); Lanes 5,6&7, ΔOmpF (JW0912) + kanamycin (0xMIC, ½x MIC and 3xMIC respectively).