Immunohistochemical staining of paraffin embedded human kidney with purified ab108362 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

All lanes : Anti-Caspase-1 antibody [EPR4321] (ab108362) at 1/1000 dilution (unpurified)Lane 1 : THP-1 cell lysatesLane 2 : THP-1+TPA+LPS cell lysateLane 3 : Jurkat cell lysateLane 4 : C6 cell lysate Lane 5 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical staining of caspase-1 in paraffin-embedded human breast invasive ductal carcinoma tissue with unpurified ab108362, at a dilution of 1/250.