All lanes : Anti-Caspase-1 antibody (ab82563) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Lung (Human) Tissue LysateLane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.