ab1872 staining Caspase-1 in mouse immortalised bone marrow-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1:200 in PBS + 0.1% BSA/5% goat serum) for 1 hour at 20°C. An anti-rabbit Alexa Fluor®568 (1/500) ab175471) was used as the secondary antibody.See Abreview