Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99313 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CAD antibody (ab99313) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of CAD by Western Blot of Immunprecipitate. ab99313 at 0.1µg/ml staining CAD in HeLa whole cell lysate immunoprecipitated using ab99313 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.