Detection of Human RAMP1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RAMP1 in Human Brain. RAMP1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal and glial processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.