Mouse/Rat Neurocan Antibody
| Name | Mouse/Rat Neurocan Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF5800 |
| Prices | $99.00, $359.00 |
| Sizes | 25 µg, 100 µg |
| Host | Sheep |
| Isotype | IgG |
| Applications | IHC WB |
| Species Reactivities | Mouse, Rat |
| Antigen | Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan Asp23-Asp637 Accession # NP_031815 |
| Purity/Format | Antigen Affinity-purified |
| Description | Sheep |
| Gene | NCAN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Mouse/Rat Neurocan by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Neurocan at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Neurocan in Mouse Brain. Neurocan was detected in perfusion fixed frozen sections of mouse brain (medulla) using Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections .
Product References
Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl - Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl
Bartels MF, Winterhalter PR, Yu J, Liu Y, Lommel M, Mohrlen F, Hu H, Feizi T, Westerlind U, Ruppert T, Strahl S. PLoS One. 2016 Nov 3;11(11):e0166119.
L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem - L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem
Ebbers L, Satheesh SV, Janz K, Ruttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang HG. J Biol Chem. 2015 Sep 25;290(39):23692-710.
Neurons and glia modify receptor protein-tyrosine phosphatase zeta - Neurons and glia modify receptor protein-tyrosine phosphatase zeta
Dwyer CA, Katoh T, Tiemeyer M, Matthews RT. J Biol Chem. 2015 Apr 17;290(16):10256-73.
Neurocan contributes to the molecular heterogeneity of the perinodal ECM. - Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Bekku Y, Oohashi T. Arch Histol Cytol. 2010;73(2):95-102.