Immunohistochemical staining of paraffin embedded human kidney with purified ab108299 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of paraffin embedded mouse kidney with purified ab108299 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of paraffin embedded rat stomach with purified ab108299 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunofluorescent staining of HeLa cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab108299 at a dilution of 1/150. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

All lanes : Anti-c-Rel antibody [EPR2558] (ab108299) at 1/1000 dilution (unpurified)Lane 1 : Daudi cell lysateLane 2 : HuT-78 cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab108299 at a working dilution of 1 in 10. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical analysis of Human diffuse B-cell lymphoma tissue using unpurified ab108299 at a dilution of 1/100.

Immunohistochemical staining of paraffin embedded mouse kidney with unpurified ab108299 at a working dilution of 1 in 10. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemical staining of paraffin embedded rat stomach with unpurified ab108299 at a working dilution of 1 in 10. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Unpurified ab108299 staining c-Rel in human macrophages by immunocytochemistry/ immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 5% goat serum for 1 hour at 37°C and then incubated with unpurified ab108299 at a 1/500 dilution for 3 hours at 37°C. The secondary used was an Alexa-Fluor® 488 conjugated goat anti-rabbit monoclonal used at a 1/1000 dilution.See Abreview

Immunofluorescent staining of HeLa cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab108299 at a dilution of 1/10. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.