CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in mouse splenocytes using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.