Human Galectin-1 Affinity Purified Polyclonal Ab, Goat IgG
| Name | Human Galectin-1 Affinity Purified Polyclonal Ab, Goat IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF1152-SP |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Simple Western WB FC IHC |
| Species Reactivities | Human |
| Antigen | E. coli-derived recombinant human Galectin‑1. Ala2-Asp135 Accession Number P09382 |
| Description | Goat Polyclonal |
| Gene | LGALS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human Galectin‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑1 in Human Prostate Cancer Tissue. Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Galectin‑1 in Human Prostate Cancer Tissue. Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of stromal cells and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Galectin‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human Galectin‑1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 17 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Product References
Galectin-1-mediated cell death is increased by CD30-induced signaling in - Galectin-1-mediated cell death is increased by CD30-induced signaling in
Suzuki O, Hirsch B, Abe M, Durkop H, Stein H. Lab Invest. 2012 Feb;92(2):191-9.
Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects - Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects
Zhuo Y, Chammas R, Bellis SL. J Biol Chem. 2008 Aug 8;283(32):22177-85.