Human Aurora A Affinity Purified Polyclonal Ab, Goat IgG
| Name | Human Aurora A Affinity Purified Polyclonal Ab, Goat IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF3295-SP |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB Simple Western |
| Species Reactivities | Human |
| Antigen | E. coli-derived recombinant human Aurora A. Met1-Glu131 Accession Number O14965 |
| Description | Goat Polyclonal |
| Gene | AURKA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human Aurora A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora A at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human Aurora A by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aurora A at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human Aurora A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3295) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.