Human/Mouse HSP27 Affinity Purified Polyclonal Ab, Rabbit IgG
| Name | Human/Mouse HSP27 Affinity Purified Polyclonal Ab, Rabbit IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF1580-SP |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Simple Western WB |
| Species Reactivities | Human, Mouse |
| Antigen | E. coli-derived recombinant human HSP27 Accession Number P04792 |
| Description | Rabbit Polyclonal |
| Gene | HSPB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human/Mouse HSP27 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human HSP27 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Detection of Mouse HSP27 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Product References
Heat shock protein 27 differentiates tolerogenic macrophages that may support - Heat shock protein 27 differentiates tolerogenic macrophages that may support
Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK. Cancer Res. 2011 Jan 15;71(2):318-27.
The heat shock response and chaperones/heat shock proteins in brain tumors: - The heat shock response and chaperones/heat shock proteins in brain tumors:
Graner MW, Cumming RI, Bigner DD. J Neurosci. 2007 Oct 17;27(42):11214-27.
Phosphatidylinositol 3-kinase/Akt plays a role in sphingosine - Phosphatidylinositol 3-kinase/Akt plays a role in sphingosine
Takai S, Tokuda H, Matsushima-Nishiwaki R, Hanai Y, Kato K, Kozawa O. J Cell Biochem. 2006 Aug 1;98(5):1249-56.