Human PDX-1/IPF1 Affinity Purified Polyclonal Ab, Goat IgG
| Name | Human PDX-1/IPF1 Affinity Purified Polyclonal Ab, Goat IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF2419-SP |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Simple Western WB ICC/IF IHC |
| Species Reactivities | Human |
| Antigen | E. coli-derived recombinant human PDX‑1/IPF1. Ala91-Arg283 Accession Number P52945 |
| Description | Goat Polyclonal |
| Gene | PDX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX‑1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Mouse PDX‑1/IPF1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Product References
FGF signaling via MAPK is required early and improves Activin A-induced - FGF signaling via MAPK is required early and improves Activin A-induced
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Generation of glucose-responsive, insulin-producing cells from human umbilical - Generation of glucose-responsive, insulin-producing cells from human umbilical
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CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived - CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived
Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H. Stem Cells. 2011 Apr;29(4):609-17.
Long-term self-renewal and directed differentiation of human embryonic stem cells - Long-term self-renewal and directed differentiation of human embryonic stem cells
Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S. Proc Natl Acad Sci U S A. 2006 May 2;103(18):6907-12. Epub 2006 Apr 21.