Neuron-specific beta-III Tubulin MAb (Clone TuJ-1), Mouse IgG2A
| Name | Neuron-specific beta-III Tubulin MAb (Clone TuJ-1), Mouse IgG2A |
|---|---|
| Supplier | R&D Systems |
| Catalog | MAB1195-SP |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2A |
| Clone | TuJ-1 |
| Applications | WB ICC/IF Simple Western |
| Species Reactivities | Broad species |
| Antigen | Rat brain-derived microtubules |
| Description | Mouse Monoclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
beta ‑III Tubulin in Differentiated Human Neural Progenitor Cells. beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195) and 10 µg/mL Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Rat beta ‑III Tubulin by Simple WesternTM. Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Product References
Genetic deletion of afadin causes hydrocephalus by destruction of adherens - Genetic deletion of afadin causes hydrocephalus by destruction of adherens
Yamamoto H, Maruo T, Majima T, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Mandai K, Takai Y. PLoS One. 2013 Nov 13;8(11):e80356.
In vivo generation of neural tumors from neoplastic pluripotent stem cells models - In vivo generation of neural tumors from neoplastic pluripotent stem cells models
Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M. Stem Cells. 2012 Mar;30(3):392-404.
Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes - Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes
Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P. PLoS One. 2012;7(4):e35892.
Communication between corneal epithelial cells and trigeminal neurons is - Communication between corneal epithelial cells and trigeminal neurons is
Oswald DJ, Lee A, Trinidad M, Chi C, Ren R, Rich CB, Trinkaus-Randall V. PLoS One. 2012;7(9):e44574.
IL-17 and VEGF are necessary for efficient corneal nerve regeneration. - IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
Li Z, Burns AR, Han L, Rumbaut RE, Smith CW. Am J Pathol. 2011 Mar;178(3):1106-16.
Developmental corneal innervation: interactions between nerves and specialized - Developmental corneal innervation: interactions between nerves and specialized
Kubilus JK, Linsenmayer TF. Invest Ophthalmol Vis Sci. 2010 Feb;51(2):782-9.
Metastable primordial germ cell-like state induced from mouse embryonic stem - Metastable primordial germ cell-like state induced from mouse embryonic stem
Yamano N, Kimura T, Watanabe-Kushima S, Shinohara T, Nakano T. Biochem Biophys Res Commun. 2010 Feb 12;392(3):311-6. doi:
A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for - A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for
Yue XS, Murakami Y, Tamai T, Nagaoka M, Cho CS, Ito Y, Akaike T. Biomaterials. 2010 Jul;31(20):5287-96.
Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of - Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of
Oh J, McCloskey MA, Blong CC, Bendickson L, Nilsen-Hamilton M, Sakaguchi DS. J Neurosci Res. 2010 Oct;88(13):2798-809.
Synovial sarcoma is a stem cell malignancy. - Synovial sarcoma is a stem cell malignancy.
Naka N, Takenaka S, Araki N, Miwa T, Hashimoto N, Yoshioka K, Joyama S, Hamada K, Tsukamoto Y, Tomita Y, Ueda T, Yoshikawa H, Itoh K. Stem Cells. 2010 Jul;28(7):1119-31.