ab112521 staining BCA1 in Rat brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal(1/1000) was used as the secondary antibody.See Abreview