Anti-Bad (phospho S112) antibody [EPR1891(2)]

Name	Anti-Bad (phospho S112) antibody [EPR1891(2)]
Supplier	Abcam
Catalog	ab129192
Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR1891(2)
Applications	WB IP
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bad
Blocking Peptide	Bad peptide
Description	Rabbit Monoclonal
Gene	BAD
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)Lane 1 : Untreated C6 cellsLane 2 : C6 cells treated with Calcyculin ALysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)Lane 1 : Untreated HeLa cellsLane 2 : HeLa cells treated with Calcyculin ALysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilutionLane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates treated with Calyculin A.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Conditional transgenic expression of PIM1 kinase in prostate induces - Conditional transgenic expression of PIM1 kinase in prostate induces

Narlik-Grassow M, Blanco-Aparicio C, Cecilia Y, Perez M, Munoz-Galvan S, Canamero M, Renner O, Carnero A. PLoS One. 2013;8(4):e60277.