Lanes 1 - 3 : Anti-BAALC antibody [7.3_1E10] (ab127977) at 1/1000 dilutionLanes 4 - 6 : V5 antibody control at 1/1000 dilutionLane 1 : Transfected HeLa cell lysateLane 2 : Non-Transfected HeLa cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : Transfected HeLa cell lysateLane 5 : Non-Transfected HeLa cell lysateLane 6 : SH-SY5Y cell lysate

2 µg of ab127977 was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded. Lane 1: Cell Lysate control Lane 2: Cell lysate with ab127977 Lane 3: Cell lysate without ab127977 Lane 4: V5 tag control