All lanes : Anti-ATR antibody (ab101900) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLane 8 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-ATR antibody (ab101900) at 1 µg/mlLane 1 : Testis (Mouse) Tissue Lysate Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.