![Anti-ATP5J antibody [10C6AC9] (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/11125_ATP5J-Primary-antibodies-ab110279-1.jpg)
Anti-ATP5J antibody [10C6AC9] (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg

Mitochondrial localization of ATP synthase visualized by immunocytochemistry using ab110279. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110279 (1 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).

HL-60 cells were stained with 1 µg/mL ab110279 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
![All lanes : Anti-ATP5J antibody [10C6AC9] (ab110279)Lane 1 : human heart homogenate at 20 µgLane 2 : HepG2 cell lysate at 20 µgLane 3 : human liver mitochondrial extraction at 10 µgLane 4 : bovine heart mitochondrial extraction at 10 µgLane 5 : human heart mitochondrial extraction at 10 µgLane 6 : human liver homogenate at 20 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/11128_ATP5J-Primary-antibodies-ab110279-6.jpg)
All lanes : Anti-ATP5J antibody [10C6AC9] (ab110279)Lane 1 : human heart homogenate at 20 µgLane 2 : HepG2 cell lysate at 20 µgLane 3 : human liver mitochondrial extraction at 10 µgLane 4 : bovine heart mitochondrial extraction at 10 µgLane 5 : human heart mitochondrial extraction at 10 µgLane 6 : human liver homogenate at 20 µg