
All lanes : Anti-ARAP3 antibody (ab5911) at 1/500 dilutionLane 1 : HEK293 negative controlLane 2 : HEK293 overexpressed GFP-ARAP3Lane 3 : Whole tissue lysate prepared from mouse heartLane 4 : Whole tissue lysate prepared from mouse brainLane 5 : Whole tissue lysate prepared from mouse diaphragmaLane 6 : Whole tissue lysate prepared from mouse fundusLane 7 : Whole tissue lysate prepared from rabbit heartLysates/proteins at 100 µg per lane.SecondaryAlexa-Fluor 680 conjugated donkey polyclonal at 1/10000 dilutionImage courtesy of an anonymous Abreview.See Abreview