All lanes : Anti-Aquaporin 1 antibody (ab87845) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816)Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.