
Anti-Apg12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

Anti-Apg12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

All lanes : Anti-Apg12 antibody (ab155589) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLysates/proteins at 30 µg per lane.

Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Apg12 with ab155589 at 1/500 dilution.

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Apg12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.Cells were fixed in 4% paraformaldehyde for 15 minutes.