Anti-Apc6 antibody [EPR16889]

Name	Anti-Apc6 antibody [EPR16889]
Supplier	Abcam
Catalog	ab181567
Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR16889
Applications	WB IP
Species Reactivities	Mouse, Rat, Human
Antigen	Recombinant fragment within Mouse APC aa 500 to the C-terminus
Description	Rabbit Monoclonal
Gene	CDC16
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilutionLane 1 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysates at 20 µgLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µgLane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates at 20 µgLane 4 : HT1080 (Human fibrosarcoma cells) whole cell lysates at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/1000 dilutionLane 1 : Human fetal liverLane 2 : Mouse liverLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysatesLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

APC6 Protein was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181567 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181567 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.