All lanes : Anti-Apc6 antibody (ab72136) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 15 µgLane 2 : Whole cell lysate from Hela cells at 5 µgLane 3 : Whole cell lysate from Hela cells at 1.5 µgLane 4 : Whole cell lysate from Hela cells at 0.5 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Immunoprecipitation/ Western Blot of Apc6. Lane 1: ab72136 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72136 at 0.1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection:Chemiluminescence with an exposure time of 30 seconds.