Anti-Androgen Receptor antibody [AN1-15]
| Name | Anti-Androgen Receptor antibody [AN1-15] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2742 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | AN1-15 |
| Applications | ICC/IF IHC-F IP WB EMSA IHC-P |
| Species Reactivities | Mouse, Rat, Human, Primate, Monkey |
| Antigen | Recombinant fragment corresponding to Human Androgen Receptor aa 331-572 |
| Description | Rat Monoclonal |
| Gene | AR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Anti-Androgen Receptor antibody [AN1-15] (ab2742) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/6826_ab2742_1.jpg)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Anti-Androgen Receptor antibody [AN1-15] (ab2742) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
ICC/IF image of ab2742 staining human prostate cells expressing androgen receptors. The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C. The primary anitibody was diluted 1/50 in 20% horse serum in PBS and incubated for 20 minutes at 4°C. An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.The blue staining is the cell nuclei, the green staining shows the androgen receptors.See Abreview
Product References
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