![All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLane 5 : HEK293 cell lysateLane 6 : HepG2 cell lysateLane 7 : NIH3T3 cell lysateLane 8 : PC12 cell lysateLane 9 : COS7 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5878_alpha-smooth-muscle-Actin-Primary-antibodies-ab119952-1.jpg)
All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLane 5 : HEK293 cell lysateLane 6 : HepG2 cell lysateLane 7 : NIH3T3 cell lysateLane 8 : PC12 cell lysateLane 9 : COS7 cell lysate
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
Immunofluorescence analysis of HepG2 cells using ab119952 at 1/200 (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (blue).
Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
ELISA, using ab119952 at a dilution of 1/10000.