Anti-alpha smooth muscle Actin antibody (ab54723) at 1 µg/ml + Whole tissue lysate prepared from human omentum, serous carcinoma.
Detection limit for GST tagged alpha smooth muscle actin is approximately 1 ng/ml as a capture antibody.
ab54723 at 1 - 10 ug/ml staining Alpha smooth muscle actin in human Hela cells by Immunocytochemistry / Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab54723 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat ant-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5854_alpha-smooth-muscle-Actin-Primary-antibodies-ab54723-2.jpg)
Overlay histogram showing HeLa cells stained with ab54723 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat ant-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.