ab108203 staining Alcohol Dehydrogenase in the HepG2 cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 1 hour at 22°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody. Nucleus was stained with DAPI (blue).See Abreview
![All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Mouse liver lysateLane 3 : Human fetal liver lysateLane 4 : Rat liver lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/4605_Alcohol-Dehydrogenase-Primary-antibodies-ab108203-5.jpg)
All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (ab108203) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Mouse liver lysateLane 3 : Human fetal liver lysateLane 4 : Rat liver lysateLysates/proteins at 10 µg per lane.
ab108203 at 1/250 dilution staining Alcohol Dehydrogenase in paraffin-embedded Human liver tissue.
ab108203 at 1/500 dilution staining Alcohol Dehydrogenase in HepG2 cells by Immunofluorescence.