Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling AKR1C1 with ab183078 at a 1/30 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
All lanes : Anti-AKR1C1 antibody - C-terminal (ab183078) at 1/500 dilutionLane 1 : Human liver cancer tissue lysateLane 2 : Human breast infiltrative duct tissue lysateLane 3 : Human fetal brain tissue lysateLysates/proteins at 27 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique