
Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : HepG2 lysateLane 3 : JAR lysateLane 4 : Human milk lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3064_ADFP-Primary-antibodies-ab108323-5.jpg)
All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : HepG2 lysateLane 3 : JAR lysateLane 4 : Human milk lysateLysates/proteins at 10 µg per lane.

ab108323 at 1/100 dilution staining ADFP in HepG2 cells by Immunofluorescence.
![All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3066_ADFP-Primary-antibodies-ab108323-6.jpg)
All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLysates/proteins at 10 µg per lane.