Anti-Adenylosuccinate Lyase antibody [EPR10746(B)]

Name	Anti-Adenylosuccinate Lyase antibody [EPR10746(B)]
Supplier	Abcam
Catalog	ab154872
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR10746(B)
Applications	WB IP ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide corresponding to residues in Human Adenylosuccinate Lyase (P30566)
Description	Rabbit Monoclonal
Gene	ADSL
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Adenylosuccinate was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1500 dilution.Lane 1: NIH/3T3 whole cell extract, 10 µg (Input).Lane 2: ab154872 IP in NIH/3T3 whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 seconds

All lanes : Anti-Adenylosuccinate Lyase antibody [EPR10746(B)] (ab154872) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : NIH/3T3 cell lysateLane 4 : HeLa cell lysateLane 5 : HepG2 cell lysateLane 6 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunofluorescence analysis of HeLa cells labeling Adenylosuccinate Lyase with ab154872 at 1/250 dilution.