
Western blot analysis of rat brain lysate: 1. Anti-TRPV4 (extracellular) antibody (#AG1440), (1:200). 2. Anti-TRPV4 (extracellular) antibody, preincubated with the control peptide antigen.

Expression of TRPV4 in rat DRG Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) antibody (# AG1440). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.

Expression of TRPV4 in human U-87 cells Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using Anti-TRPV4 (extracellular) antibody (#AG1440), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).

Expression of TRPV4 in human U-87 cells Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) antibody (#AG1440), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.