Anti-ADAM17 antibody (ab13535) at 0.2 µg/ml + Hela lysate (RIPA buffer, 35µg total protein per lane)
HT1080 cells incubated with ab13535 at 10µg/ml for 1 hour at 22°C. Cells were fixed for 20 minutes in 4% PFA in PBS then permeabilised in 0.1% Triton for 4 minutes
ab13535 Immunoprecipitate in human HEK293 whole cell lysate. 2 x 106 cells were used to prepare lysates and then incubated with primary antibody (diluted 1/250) and matrix (Protein G) for 16 hours at 4°C. For western blotting an Abcam ab28233 Rabbit polyclonal to ADAM17 was used at dilution at 1/5000.See Abreview