Immunohistochemical analysis of paraffin-embedded H. kidney section using ECHDC3 Antibody (N-term)(Cat#AP20684a). AP20684a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from human liver and kidney tissue lysate (from left to right), using ECHDC3 Antibody (N-term)(Cat. #AP20684a). AP20684a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.