Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (C-term)(green, Cat#AP20582c) compared to an isotype control of rabbit IgG(blue). AP20582c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody (C-term)(Cat#AP20582c). AP20582c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody (C-term)(Cat#AP20582c). AP20582c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from human brain, mouse brain and eyeball tissue lysate (from left to right), using HBG2 Antibody (C-term) (Cat. #AP20582c). AP20582c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.