All lanes : Anti-ACOT7 antibody [EPR11318(B)] (ab157472) at 1/10000 dilutionLane 1 : Fetal brain lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Anti-ACOT7 antibody [EPR11318(B)] (ab157472) at 1/10000 dilution + immunoprecipitation pellet from Jurkat cell lysateSecondaryHRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG
Flow cytometric analysis of permeabilized 293T cells labelling ACOT7 with ab157472 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).