All lanes : Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1000 dilution (unpurified)Lane 1 : Mouse kidneyLane 2 : Rat kidneyLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1000 dilution (unpurified) + HepG2 cell lysateSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1500 dilution (purified) + Human fetal kidney at 10 µgSecondaryHRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1000 dilution (unpurified) + Human fetal kidney at 10 µgSecondaryHRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescence staining of HepG2 cells with purified ab126595 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab126595 was used at a dilution of 1/250 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

All lanes : Anti-Aconitase 1 antibody [EPR7225] (ab126595) at 1/1000 dilution (unpurified)Lane 1 : Human thyroid lysateLane 2 : HeLa cell lysateLane 3 : Human fetal kidney lysateLane 4 : HepG2 cell lysateLane 5 : T.T. cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Unpurified ab126595 at 1/100 dilution staining Aconitase 1 in HeLa cells by immunofluorescence.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD