All lanes : Anti-Acetyl-CoA acetyltransferase [EPR8416(2)] antibody (ab191431) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab191431 at 1/20 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes : Anti-Acetyl-CoA acetyltransferase [EPR8416(2)] antibody (ab191431) at 1/1000 dilutionLane 1 : PC12 cell lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution