Anti-acetyl Lysine antibody - ChIP Grade
| Name | Anti-acetyl Lysine antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab21623 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ChIP ICC/IF ICC/IF WB ELISA |
| Antigen | Acetylated KLH conjugates |
| Description | Rabbit Polyclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Primary: All Lanes: Anti acetyl Lysine antibody (ab21623) at 1:1000. Lane 1: Marker. Lane 2: HeLa cells vehicle-treated (ab139414). Lane 3: HeLa cells, trichostatin A-treated (ab139414). Lysates at 20 ug/lane . Secondary: All Lanes: Goat anti-Rabbit IgG 1:10000. Performed under reducing conditions. Blocking buffer: 5% milk in PBS. Observed band sizes: 11 kDa 15kDa 45kDa 50 kDa.
Lane 1 = Extract of Mcf7 cells incubated with vehicle ? 20 ug. Lane 2 = Extract of Mcf7 cells incubated with trichostatin A ? 20 ug. Lane 3 = Extract of Mcf7 cells incubated with EX527 ? 20 ug. Lane 4 = Extract of Mcf7 cells incubated with nicotinamide ? 20 ug. Lane 5 = Extract of Mcf7 cells incubated with camptothecin ? 20 ug. Lane 6 = Extract of Mcf7 cells incubated with camptothecin and trichostatin A ? 20 ug. Lane 7 = Extract of Mcf7 cells incubated with camptothecin and EX527 ? 20 ug. Lane 8 = Extract of Mcf7 cells incubated with camptothecin and nicotinamide ? 20 ug. Lane 9 = Extract of 293T cells incubated with vehicle ? 20 ug. Lane 10 = Extract of 293T cells incubated with camptothecin ? 20 ug. Lane 11 = Extract of 293T cells incubated with camptothecin and trichostatin A ? 20 ug. Lane 12 = Extract of 293T cells incubated with camptothecin and EX527 ? 20 ug. Lane 13 = Extract of 293T cells incubated with camptothecin and nicotinamide ? 20 ugSDS PAGE performed under reducing con
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab21623 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-acetyl Lysine antibody - ChIP Grade (ab21623) at 0.5 µg/mlLane 1 : Untreated Human melanoma cell lysateLane 2 : TSA-treated Human melanoma cell lysateLysates/proteins at 75 µg per lane.SecondaryGoat anti-rabbit IgG HRP at 0.25 µg/mldeveloped using the ECL techniqueObserved band size : 16-18 kDa (why is the actual band size different from the predicted?)Additional bands at : 12-14 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution.1: Untreated cells2: TSA treated cells
p53 acetylation upon Doxorubicin treatment in human melanoma cells (MMRU cells). MMRU cells were treated with 0.5 ug/ml Dox for various times and lyzed for whole protein. Immunoprecipitation was performed with ab21623. Western blot was performed to detect the immunoprecipitated p53 with anti-p53 antibody.
Product References
Histone deacetylase inhibition reduces cardiac connexin43 expression and gap - Histone deacetylase inhibition reduces cardiac connexin43 expression and gap
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Substrate and functional diversity of lysine acetylation revealed by a proteomics - Substrate and functional diversity of lysine acetylation revealed by a proteomics
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