ab114544 staining AADACL1 in cultured human prostate cells by ICC (Immunocytochemistry). Cells were fixed with methanol and blocked with 1% BSA for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 37°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.See Abreview

ab111544 staining AADACL1 in Human Prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA + 2% serum for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking agent) for 2 hours at 37°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview