Anti-6X His tag® antibody - ChIP Grade
| Name | Anti-6X His tag® antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab9108 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P ICC/IF ICC/IF ELISA ChIP FC |
| Antigen | Synthetic peptide: HHHHHH conjugated to KLH |
| Description | Rabbit Polyclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/4000 dilutionLane 1 : HeLa whole cell lysate - transfected with empty vectorLane 2 : HeLa whole cell lysate - transfected with His-tagged geneLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 35 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewSee Abreview
Use of ab9108 in western blotting at 1:1000 against Epitope Tag Positive Control lysate (ab4524). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate
All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilutionLane 1 : 10ug non-transformed lysateLane 2 : 10ug non-transformed lysate + 1mM IPTGLane 3 : 10ug E.coli Lysate harbouring His-tag-RelA NTD, non-inducedLane 4 : 10ug Induction of His-tag RelA NTD 1 mM IPTGLane 5 : 10ug Induction of His-tag RelA NTD 1 mM IPTGSecondaryHRP conjugated goat anti-rabbit IgGdeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 37 kDa (why is the actual band size different from the predicted?)Additional bands at : 12 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an Abreview.See Abreview
Product References
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