All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilutionLane 1 : 293 cells, untransfected controlLane 2 : 293 cells transfected with His-tagged vector

Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µgSecondaryAn HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview

ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).See Abreview

Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa) Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer. Lanes 6-7 : Purified PDCD5 from E. coli with ~60-70 ng protein. The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa). Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.See Abreview