Anti-5T4 antibody [EPR5530]

Name	Anti-5T4 antibody [EPR5530]
Supplier	Abcam
Catalog	ab129058
Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR5530
Applications	WB IP
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 5T4 aa 350-450 (intracellular)
Description	Rabbit Monoclonal
Gene	TPBG
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified) + Rat brain at 20 µgSecondaryHRP goat-anti rabbit (H+L) at 1/1000 dilution

Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified) + Rat brain at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified)Lane 1 : HT-1376 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified)Lane 1 : HT-1376 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/1000 dilution (unpurified)Lane 1 : HT 1376 cell lysateLane 2 : HeLa cell lysateLane 3 : JAR cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.